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Product Name:

DNA-RNA Hybrid Monoclonal Antibody [S9.6]

Cat No.:

2112

Clonality:

Monoclonal

Species Reactivity:

All Species

Tested Applications:

ELISA, IP, IF, CHIP, Dot Blot

Recommended Dilution:

IP: 1:50-500; IF: 1: 200

Format:

Liquid

Source:

Mouse

Purification Method:

Protein A/G Purified

Isotype:

IgG2a

Conjugate:

Unconjugated

Storage:

Store at -20℃. Do not aliquot

Immunogen:

ΦX174 bacteriophage-derived synthetic DNA/RNA

Full Name:

DNA-RNA Hybrid

Background:

The DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. The S9.6 monoclonal antibody recognizes DNA-RNA hybrids (also known as R-loops) and does not bind to single or double stranded DNA. The antibody has high affinity for DNARNA hybrids but also binds RNA-RNA hybrids that are AU-rich. The specificity of the antibody appears to be determined by a combination of sequence and structural dependency since Rloop sequence affects binding affinity.

Category:

Primary Ab