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ChromoTek GFP-Trap? Agarose
| 貨號(hào) | gta | 售價(jià)（元） | 4350 |
| 規(guī)格 | 20rxns | CAS號(hào) |
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Product Information：
GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.
Description：
Immunoprecipitation of GFP-fusion proteins and their interacting factors with anti-GFP Nanobody conjugated to beads.
• Fast, reliable & efficient one-step immunoprecipitation
• Ready-to-use
• No heavy & light antibody chains
• Stable under harsh washing conditions
• Suitable for downstream mass spec analysis
• Works in samples from: mammals, plants, bacteria, yeast, insects etc.
Applications：IP, CoIP, ChIP, RIP
Specificity/Target：AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP.For the complete list, please click here: Fluorescent protein specificity table
Binding capacity：25-30 µg of recombinant GFP per 25 µL bead slurry
Conjugate：Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution buffer：
SDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility：
1 mM DTT,
3 M Guanidinium•HCl, 8 M Urea,
2 M NaCl,
2 % Nonidet P40 Substitute,
1 % SDS,
1 % Triton X-100
Type：Nanobody
Class：Recombinant
Host：Alpaca
Affinity (KD)：Dissociation constant KD of 1 pM
Compatibility with mass spectrometry：The GFP-Trap® is optimized for on-bead digestion. On-bead digest protocol for mass spectrometry
RRID：AB_2631357
Storage Buffer：20% ethanol
Storage Condition：Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!