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5-formylcytosine polyclonal antibody
| 貨號 | C15310200 | 售價（元） | 咨詢 |
| 規(guī)格 | 100ul | CAS號 |
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Polyclonal antibody raised in rabbit against 5-formylcytosine (5-fC) conjugated to KLH.
| Lot | A1941-001 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human, mouse, other (wide range) |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| DIP | 3 μl/IP | Fig 1 |
| ELISA | 1:10,000 | Fig 2 |
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Validation Data
Figure 1. DIP results obtained with the Diagenode antibody directed against 5-fC
HEK293 cells were transfected with a reporter gene and hydroxymethylated in vitro with either a pCAG expression vector containing the TET2 catalytic domain (TET2cd) or a negative control pCAG vector. DIP assays were performed on 4 μg of sheared and denatured DNA using 3 μl of the Diagenode antibody against 5-fC (Cat. No. C15310200) in a total of 500 μl IP buffer. QPCR was performed with primers specific for the reporter gene. Figure 1 shows the recovery, expressed as a % of input (mean +standard deviation of 3 different experiments).
Figure 2. Determination of the titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against 5-fC (Cat. No. C15310200). The plates were coated with the immunogen. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be >1:100,000.