午夜国产av新品一区二区,久久久婷婷精品福利国产午夜,亚洲乱码美女一区二区三区,色综合久久伊人v,人妻丰满熟妇aⅴ无码区,日本一区二区免费在线观看视频,国产黄色一级片免费在线观看,精品久久久久久久国产懂色av

Product uses:

Measurement of microtubule-activated ATPase activity

Identification/characterization of proteins or small molecules that affect motor ATPase activity

Identification/characterization of proteins or small molecules that affect kinesin motility

Identification/characterization of proteins or small molecules that affect motor/microtubule interactions

Material：
       The conserved motor domain of human MKLP1 was expressed in a prokaryotic system. The recombinant protein contains a GSTTag at the amino terminal end and has a combined molecular weight of 82 kD. The protein has been determined to be biologically active in a microtubule-activated ATPase activity test. The protein is supplied as a lyophilized powder.

Purity：
       Protein purity is estimated by scanning densitometry of a coomassie-stained SDS-PAGE gradient gel. Figure 1 in the datasheet shows 10 ug of MP01 protein and purity was determined to be >70%. The total protein in each tube will therefore be approximately 30% greater than the amount shown on the tube. The major contaminant at approximately 30 kD is GST protein. The microtubule-activated ATPase activity of the MKLP1 motor is not inhibited by this contaminant. .

Biological Activity - Microtubule Activated ATPase Assay：
       MKLP1 ATPase activity was measured by monitoring real time free phosphate generation using the Kinesin ELIPA Assay Kit (cat.# BK060). The assay is based upon an absorbance shift (330 nm - 360 nm) that occurs when 2-amino-6-mercapto-7methylpurine ribonucleoside (MESG) is catalytically converted to 2-amino-6-mercapto-7-methylpurine in the presence of inorganic phosphate (Pi). One molecule of Pi will yield one molecule of 2amino-6-mercapto-7-methylpurine in an essentially irreversible reaction. Hence, the absorbance at 360 nm is directly proportional to the amount of Pi generated in the kinesin ATPase reaction. Under the conditions outlined below, the Vmax for MKLP1 microtubule-activated ATPase activity for this Lot was 1000 nmoles ATP generated per minute per mg of MP01 (Figure 2). The ATPase rate for this Lot using a 10 minute endpoint assay (Kinesin ATPase End Point Assay Kit, cat.# BK053) was 600 nmoles ATP per minute per mg of MP01. Both of these values are above the guaranteed minimum.

       Figure 2. MP01 microtubule-activated ATPase activity using Kinesin ELIPA Assay Kit (Cat.# BK060).